GEO series
Single Nuclei and Spatial Transcriptomic Datasets in Brain Metastases
GSE325196
Homo sapiens
Other; Expression profiling by high throughput sequencing
35 samples
Submitted 2026/05/23
Platform GPL24676
Summary
We performed single nuclei and spatial transcriptomic sequencing on a cohort of solid tumor human brain metastasis specimens resected at Duke University Medical Center from 1998 to 2022. This includes 23 single nuclei specimens, 12 Visium standard specimens, and 4 Xenium specimens. These samples encompass metastases from lung cancer, breast cancer, melanoma, and sarcoma primaries. Analysis of these data revealed novel tumor-microenvironment cell-cell interactions associated with patient survival.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE325196_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 35 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1438986 and SRA study SRP684746. Searching any of these in the dataset finder brings you back here.
Study design
35 conditions, each sampled once — no replicated groups
Read from 35 sample titles: 35 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9597702 Single nuclei 07-0649 specimen
- GSM9597703 Single nuclei 09-0493 specimen
- GSM9597704 Single nuclei 09-0602 specimen
- GSM9597705 Single nuclei 10-0264 specimen
- GSM9597706 Single nuclei 11-0092 specimen
- GSM9597707 Single nuclei 11-0418 specimen
- GSM9597708 Single nuclei 11-0430 specimen
- GSM9597709 Single nuclei 12-0235 specimen
- GSM9597710 Single nuclei 13-0140 specimen
- GSM9597711 Single nuclei 14-0124 specimen
- GSM9597712 Single nuclei 14-0228 specimen
- GSM9597713 Single nuclei 15-0065 specimen
- GSM9597714 Single nuclei 15-0112 specimen
- GSM9597715 Single nuclei 15-0143 specimen
- GSM9597716 Single nuclei 15-0215 specimen
- GSM9597717 Single nuclei 16-0065 specimen
- GSM9597718 Single nuclei 16-0144 specimen
- GSM9597719 Single nuclei 17-0250 specimen
- GSM9597720 Single nuclei 18-0126 specimen
- GSM9597721 Single nuclei 18-0137 specimen
- GSM9597722 Single nuclei 19-0016 specimen
- GSM9597723 Single nuclei 19-0145 specimen
- GSM9597724 Single nuclei 19-0324 specimen
- GSM9597725 Visium standard 10-0264 frozen specimen
- GSM9597726 Visium standard 11-0418 FFPE specimen
- GSM9597727 Visium standard 15-0065 FFPE specimen
- GSM9597728 Visium standard 15-0215 FFPE specimen
- GSM9597729 Visium standard 16-0144 frozen specimen
- GSM9597730 Visium standard 16-0239 frozen specimen
- GSM9597731 Visium standard 18-0126 FFPE specimen
- GSM9597732 Visium standard 19-0016 frozen specimen
- GSM9597733 Visium standard 19-0145 frozen specimen
- GSM9597734 Visium standard 19-0145 FFPE specimen
- GSM9597735 Visium standard 19-0170 frozen specimen
- GSM9597736 Visium standard 16-0239 FFPE specimen
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