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ATAC-seq of the granulopoiesis lineage from different organs

GSE318435 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 34 samples Submitted 2026/04/17 Platform GPL24247
Summary
We performed ATAC-seq of 7,000-50,000 ex-vivo flow-sorted cells from the granulopoietic tree in mice. For this we sorted Granulocyte-Macrophage Progenitors (GMP) from bone marrow (BM) and spleen (SP), Pre-Neutrophils (PreN) from BM and SP as well as Immature Neutrophils (imNeu) and Mature neutrophils (Neutro) from BM, SP and Blood (Bld). After sorting, cells were immediately processed using the OmniATAC protocol (Corces, Nat Methods 2014) and sequenced on a Novaseq-6000.
Published in
Divergent granulopoiesis at extramedullary sites safeguards antibacterial host defense
Silvestre-Roig C, Chevre R, Farjia M et al. · Science immunology 2026 · PMID 41996475 · doi:10.1126/sciimmunol.adw7077
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Direct links to NCBI, no account and no request form: the whole study as GSE318435_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 34 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1418729 and SRA study SRP674636. Searching any of these in the dataset finder brings you back here.

Study design
10 conditions, mostly with about 4 replicates each
PreN,BM, ×4 PreN,SP, ×4 imNeu,BM, ×4 imNeu,SP, ×4 Neutro,BM, ×4 Neutro,SP, ×4 imNeu,Bld, ×3 Neutro,Bld, ×3 +2 more

Supports a between-group comparison across 34 samples.

10 replicated groups read from 34 sample titles; they account for 34 of them. Check it against the sample list below before relying on it.

Samples in this study
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