GEO series
Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation [ChIP-seq]
GSE300575
Homo sapiens
Genome binding/occupancy profiling by high throughput sequencing
22 samples
Submitted 2026/07/07
Platform GPL18573
Summary
Cohesin organizes 3D chromatin architecture, including promoter–enhancer loops, yet its loss has surprisingly modest effects on steady-state gene expression. We address this paradox by demonstrating that cohesin acts at different stages of transcription in human cells. First, it promotes Pol II promoter recruitment by facilitating enhancer–promoter communication that maintains active promoter chromatin states. Second, it delays pause release by transiently associating with the transcriptional machinery during the pause–release transition. Kinetic modelling suggests that reduced Pol II recruitment and enhanced pause release have compensatory effects, contributing to minimal changes in steady-state gene expression across genes upon cohesin loss. In contrast, cohesin depletion impairs robust transcriptional induction in response to external stimuli. Moreover, cohesin ensures sufficient pausing duration as a quality-control-like step to promote elongation complex assembly and transcription processivity. Here, we show that cohesin regulates multiple transcriptional steps, offering mechanistic insight into cohesin-related diseases, including cancers and cohesinopathies.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE300575_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 22 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1281419 and SRA study SRP594172. Searching any of these in the dataset finder brings you back here.
Study design
22 conditions, each sampled once — no replicated groups
Read from 22 sample titles: 22 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
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- GSM9064733 ChIPseq, BRD4, ctrl
- GSM9064734 ChIPseq, BRD4, aux
- GSM9064735 ChIPseq, MED1, ctrl
- GSM9064736 ChIPseq, MED1, aux
- GSM9064737 ChIPseq, Input, RPB1, control
- GSM9064738 ChIPseq, Input, RPB1, DRB
- GSM9064739 ChIPseq, Input, RPB1, release
- GSM9064740 ChIPseq, RPB1, control
- GSM9064741 ChIPseq, RPB1, DRB
- GSM9064742 ChIPseq, RPB1, release
- GSM9064743 ChIPseq, Input, control
- GSM9064744 ChIPseq, Input, DRB
- GSM9064745 ChIPseq, Input, release
- GSM9064746 ChIPseq, RAD21, control
- GSM9064747 ChIPseq, RAD21, DRB
- GSM9064748 ChIPseq, RAD21, release
- GSM9064749 ChIPseq, NIPBL, control
- GSM9064750 ChIPseq, NIPBL, DRB
- GSM9064751 ChIPseq, NIPBL, release
- GSM9064752 ChIPseq, MAU2, control
- GSM9064753 ChIPseq, MAU2, DRB
- GSM9064754 ChIPseq, MAU2, release
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