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TRIM33 loss reduces Androgen Receptor transcriptional output and H2BK120 ubiquitination [ChIP-seq]

GSE284519 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 93 samples Submitted 2025/06/10 Platform GPL24676Platform GPL20301
Summary
The Androgen Receptor (AR) is a ligand-dependent transcription factor that drives prostate cancer development and progression. Although, a detailed effect on AR biology has been described for a number of interacting proteins, many AR coregulators remain to be characterized in relation to their distinct impact on AR function. Here, we describe TRIM33 as a conserved AR-interactor across multiple prostate cancer cell lines. We observed that TRIM33 and AR share overall chromatin interaction profiles, in which TRIM33 is involved in downstream responsive transcriptomic output. In contrast to prior reports, we show that TRIM33 does not impact AR protein stability, but instead propose a model in which TRIM33 facilitates maximal AR activity by interfering with H2BK120 ubiquitination levels.
Published in
TRIM33 loss reduces androgen receptor transcriptional output and H2BK120 ubiquitination
Eickhoff N, Janetzko J, Padrão N et al. · Communications biology 2025 · PMID 40646276 · doi:10.1038/s42003-025-08449-2
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Direct links to NCBI, no account and no request form: the whole study as GSE284519_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 93 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1199461 and SRA study SRP552092. Searching any of these in the dataset finder brings you back here.

Study design
12 conditions, mostly in triplicate
TRIM24_LNCaP_4hDMSO ×3 TRIM24_LNCaP_4hR1881 ×3 TRIM24_LNCaP_24hR1881 ×3 TRIM33_LNCaP_4hDMSO ×3 TRIM33_LNCaP_4hR1881 ×3 TRIM33_LNCaP_24hR1881 ×3 H3K18ac_LNCaP_4hDMSO ×3 H3K18ac_LNCaP_4hR1881 ×3 +8 more

Supports a between-group comparison across 36 samples.

12 replicated groups read from the first 40 of 93 sample titles; they account for 36 of them. Check it against the sample list below before relying on it.

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