GEO series

Prefrontal-raphe-habenular circuit drives fear memory recall and updating

GSE300145 Mus musculus Expression profiling by high throughput sequencing 38 samples Submitted 2026/06/30 Platform GPL24247

Summary

Fear memory recall is a vital defensive response, yet its underlying neuronal mechanism remains incompletely understood. Here, we identify a fear recall pathway. We found that hippocampal and amygdalar memory centers directly target a group of medial prefrontal cortex (mPFC) neurons, which are necessary for fear recall and directly target vesicular glutamate transporter 2 (vGluT2)-expressing neurons in the pontine median raphe region (MRRvGluT2 neurons) in mice. We found that MRRvGluT2 neurons are necessary for recalling contextual and cued fear memories, they exhibit scalable fear-dependent activity, bidirectionally regulate depression-like behaviors, and show prolonged activity after a fearful experience. During fear memory recall, MRRvGluT2 neurons activate the lateral habenula (LHb), a region implicated in major depression. MRRvGluT2 neurons are electrophysiologically and genetically diverse, and present in rodent and primate species. The identified mPFC–MRR–LHb pathway critically mediates fear memory recall, providing potential therapeutic targets for fear-related disorders.

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Direct links to NCBI, no account and no request form: the whole study as GSE300145_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 38 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1279125 and SRA study SRP592940. Searching any of these in the dataset finder brings you back here.

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Study design

38 × MRR,

Supports a between-group comparison across 38 samples.

1 replicated groups read from 38 sample titles; they account for 38 of them. Check it against the sample list below before relying on it.

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