GEO series
Tumor-resident T cells and dendritic cells form an in situ archetype for immunotherapy response in melanoma [bulk RNA-seq]
GSE294272
Homo sapiens
Expression profiling by high throughput sequencing
29 samples
Submitted 2026/06/08
Platform GPL24676
Summary
Tumor-resident (TR) T cells participate in immunosurveillance of melanoma, but their role in determining response to immune checkpoint inhibitors (ICI) has not been comprehensively explored. We performed spatial and single-cell profiling on 32 metastatic melanoma lymph node samples, from treatment-naïve, ICI-resistant, or ICI-responsive patients. In responders, we found enrichment for early differentiation (TCF1+) CD8+ and CD4+ TR which co-localized with melanoma cells. CD8+ TR were hyperexpanded, and both CD8+ and CD4+ TR upregulated cytotoxicity-related gene expression, suggesting functional anti-tumor immunity. Conversely, ICI-resistant tumors displayed chronic IFN-γ responses within the tumor microenvironment to potentiate T cell exhaustion. In ICI-responsive melanoma tumors, CD8⁺ TR interact with CD4⁺ TR cells (via the CCL4-CCR5 axis) and engage with type-3 dendritic cells (DC3) (via IL15-IL15R interaction). This intratumoral immune triad (CD8⁺ TR – CD4⁺ TR – DC) is exclusive to responders. This study illustrates the role of TR cells and their crosstalk with DCs in effective ICI responses.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE294272_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 29 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1248920. Searching any of these in the dataset finder brings you back here.
Study design
29 conditions, each sampled once — no replicated groups
Read from 29 sample titles: 29 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8901585 HM002_RNA-seq
- GSM8901586 HM003-Large_RNA-seq
- GSM8901587 HM003-Small_RNA-seq
- GSM8901588 HM004_RNA-seq
- GSM8901589 HM005_RNA-seq
- GSM8901590 HM006_RNA-seq
- GSM8901591 HM007_RNA-seq
- GSM8901592 HM009_RNA-seq
- GSM8901593 HM012_RNA-seq
- GSM8901594 HM014_RNA-seq
- GSM8901595 HM015_RNA-seq
- GSM8901596 HM017_RNA-seq
- GSM8901597 HM019_RNA-seq
- GSM8901598 HM020_RNA-seq
- GSM8901599 HM025_RNA-seq
- GSM8901600 HM026_RNA-seq
- GSM8901601 HM028_RNA-seq
- GSM8901602 HM030_RNA-seq
- GSM8901603 HM032_RNA-seq
- GSM8901604 HM035_RNA-seq
- GSM8901605 HM036_RNA-seq
- GSM8901606 HM038_RNA-seq
- GSM8901607 HM041_RNA-seq
- GSM8901608 Mel-TIL-002_RNA-seq
- GSM8901609 Mel-TIL-011_RNA-seq
- GSM8901610 Mel-TIL-019_RNA-seq
- GSM8901611 Mel-TIL-022_RNA-seq
- GSM8901612 Mel-TIL-026_RNA-seq
- GSM8901613 Mel-TIL-030_RNA-seq
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