GEO series

Modelling mouse embryogenesis from chemically induced totipotent stem cells

GSE292862 Mus musculus Expression profiling by high throughput sequencing 22 samples Submitted 2026/08/08 Platform GPL34328

Summary

The ability to faithfully initiate embryogenesis from a single, induced non-germline cell type, akin to the totipotent blastomeres, remains a significant challenge in developmental and synthetic biology. In this study, we established conditions that enabled the continuous ex utero development of mouse embryo models (EMs), starting solely from the very beginning of chemically induced totipotent-like stem cells (ciTotiSCs) and spanning the pre-implantation morula, blastocysts, and post-gastrulation stages with both embryonic and extraembryonic compartments. These ciTotiSCs self-organized into blastocyst-like structures that closely resembled natural blastocysts in both morphology and lineage segregation. The resulting ciTotiSC-derived EMs (ciToti-EMs) successfully progressed through key developmental milestones, including gastrulation, embryonic axis formation, hematopoiesis and early organogenesis, closely resembling E8.5 natural embryos (NEs). Furthermore, extended culture led to the development of functional cardiac tissues in ciToti-EMs, highly comparable to that of E10.5-NEs. These findings demonstrate an extraordinary ability of a single induced cell type, ciTotiSCs, to autonomously recapitulate the complete spectrum of early mouse embryogenesis. Our ciToti-EM system provides a powerful new model for investigating mammalian embryonic development and opens new avenues for creating multicellular organisms from non-germline cells.

This dataset

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Direct links to NCBI, no account and no request form: the whole study as GSE292862_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 22 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1241770 and SRA study SRP573569. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

15 conditions, each sampled once — no replicated groups

ciToti ×5 ciToti8 ×3 ciToti10 ×2

Read from 22 sample titles: 15 distinct titles with little repetition. Check it against the sample list below before relying on it.

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