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ZNF296 drives immune evasion in epithelial cancer cells [RNA-seq]

GSE283056 Homo sapiens; Mus musculus Expression profiling by high throughput sequencing 10 samples Submitted 2025/09/06 Platform GPL24676Platform GPL24247
Summary
Using a genome-wide CRISPR activation screen, we identified ZNF296, a transcription factor prominently expressed in epithelial cancers, as a key regulator of tumor resistance to NK cell-mediated cytotoxicity. To systematically investigate the mechanisms by which ZNF296 suppresses NK cell-mediated killing, RNA-seq analysis was performed on A549 cells overexpressing ZNF296 (ZNF296-OE), and 4T1 cells with knockdown of its murine homolog Zfp296 (Zfp296KD). By analyzing differential gene expression and signaling pathways, we aimed to understand the influence of ZNF296/Zfp296 on tumor-intrinsic mechanisms.
Published in
ZNF296 Drives Immune Evasion in Epithelial Cancers by Repressing Immune Stimulatory Genes
Wang H, Zhao F, Li Y et al. · Cancer research 2026 · PMID 40991281 · doi:10.1158/0008-5472.CAN-25-0153
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Direct links to NCBI, no account and no request form: the whole study as GSE283056_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 10 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1191517 and SRA study SRP548099. Searching any of these in the dataset finder brings you back here.

Study design
3 × A549 cells, Control vs 3 × A549 cells, ZNF296OE vs 2 × 4T1 cells, sgNTC vs 2 × 4T1 cells, sgZfp296-1

Supports a between-group comparison across 10 samples.

4 replicated groups read from 10 sample titles; they account for 10 of them. Check it against the sample list below before relying on it.

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