GEO series
Polyamine-dependent metabolic shielding regulates alternative splicing
GSE255769
Homo sapiens; Mus musculus
Expression profiling by high throughput sequencing
144 samples
Submitted 2025/11/11
Platform GPL16791Platform GPL24247Platform GPL24676
Summary
Metabolites are central to cellular homeostasis. Whereas much emphasis has been placed on their relevance to meet energetic and biosynthetic demands, metabolic intermediates also function as signaling molecules. Here we show that polyamines, small polycations critical to cellular homeostasis1-3, regulate the process of alternative pre-mRNA splicing. We find that the inhibition of polyamine synthesis increases the phosphorylation of spliceosomal proteins, concomitant with a remarkable perturbation of alternative splicing in cells and tissues. Mechanistically, molecular modeling combined with biochemical assays revealed that polyamines bind to acidic phosphorylatable motifs in splicing factors of the U2 snRNP SF3 subcomplex, thus preventing the action of kinases. The molecular process through which polyamines regulate protein phosphorylation is a phenomenon that we define as “metabolic shielding”.
Published in
Zabala-Letona A, Pujana-Vaquerizo M, Martinez-Laosa B et al.
· Nature 2026
· PMID 41535471
· doi:10.1038/s41586-025-09965-1
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE255769_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 144 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1076496 and SRA study SRP489717. Searching any of these in the dataset finder brings you back here.
Study design
8 conditions, mostly with about 4 replicates each
Gastrocnemius, Veh, ×5
DU145 shAMD1 cells, DOX 48 hours, plate ×4
DU145 shAMD1 cells, noDOX, plate ×4
DU145 shAMD siSCR cells, noDOX, plate ×4
DU145 shAMD siSCR cells, DOX, plate ×4
DU145 shAMD siF3A3 cells, noDOX, plate ×4
DU145 shAMD siF3A3 cells, DOX, plate ×4
Gastrocnemius, SAM486A, ×3
+8 more
Supports a between-group comparison across 32 samples.
8 replicated groups read from the first 40 of 144 sample titles; they account for 32 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8079186 DU145 shAMD1 cells, DOX 48 hours, plate 1
- GSM8079187 DU145 shAMD1 cells, DOX 48 hours, plate 2
- GSM8079188 DU145 shAMD1 cells, DOX 48 hours, plate 3
- GSM8079189 DU145 shAMD1 cells, DOX 48 hours, plate 4
- GSM8079190 DU145 shAMD1 cells, noDOX, plate 1
- GSM8079191 DU145 shAMD1 cells, noDOX, plate 2
- GSM8079192 DU145 shAMD1 cells, noDOX, plate 3
- GSM8079193 DU145 shAMD1 cells, noDOX, plate 4
- GSM8079194 DU145 shAMD1 cells, DOX 48 hours, plate 1 [single]
- GSM8079195 DU145 shAMD1 cells, DOX 48 hours, plate 2 [single]
- GSM8079196 DU145 shAMD1 cells, DOX 48 hours, plate 3 [single]
- GSM8079197 DU145 shAMD1 cells, DOX 48 hours, plate 4 [single]
- GSM8079198 DU145 shAMD1 cells, noDOX, plate 1 [single]
- GSM8079199 DU145 shAMD1 cells, noDOX, plate 2 [single]
- GSM8079200 DU145 shAMD1 cells, noDOX, plate 3 [single]
- GSM8079201 DU145 shAMD1 cells, noDOX, plate 4 [single]
- GSM8079202 DU145 shAMD siSCR cells, noDOX, plate 1
- GSM8079203 DU145 shAMD siSCR cells, noDOX, plate 2
- GSM8079204 DU145 shAMD siSCR cells, noDOX, plate 3
- GSM8079205 DU145 shAMD siSCR cells, noDOX, plate 4
- GSM8079206 DU145 shAMD siSCR cells, DOX, plate 1
- GSM8079207 DU145 shAMD siSCR cells, DOX, plate 2
- GSM8079208 DU145 shAMD siSCR cells, DOX, plate 3
- GSM8079209 DU145 shAMD siSCR cells, DOX, plate 4
- GSM8079210 DU145 shAMD siF3A3 cells, noDOX, plate 1
- GSM8079211 DU145 shAMD siF3A3 cells, noDOX, plate 2
- GSM8079212 DU145 shAMD siF3A3 cells, noDOX, plate 3
- GSM8079213 DU145 shAMD siF3A3 cells, noDOX, plate 4
- GSM8079214 DU145 shAMD siF3A3 cells, DOX, plate 1
- GSM8079215 DU145 shAMD siF3A3 cells, DOX, plate 2
- GSM8079216 DU145 shAMD siF3A3 cells, DOX, plate 3
- GSM8079217 DU145 shAMD siF3A3 cells, DOX, plate 4
- GSM8079218 Gastrocnemius, Veh, 1
- GSM8079219 Gastrocnemius, Veh, 2
- GSM8079220 Gastrocnemius, Veh, 3
- GSM8079221 Gastrocnemius, Veh, 4
- GSM8079222 Gastrocnemius, Veh, 5
- GSM8079223 Gastrocnemius, SAM486A, 1
- GSM8079224 Gastrocnemius, SAM486A, 2
- GSM8079225 Gastrocnemius, SAM486A, 3
+ 104 more — browse all 144 samples with per-sample file links →
Similar datasets
- GSE286557 Nitric oxide drives proteomic diversity through alternative splicing 44 samples
- GSE288077 Metabolic dysfunction-associated steatotic liver disease may increase intrahepatic interferon gene signatures in patients with chronic hepatitis B 35 samples
- GSE289420 Astrocyte-derived cholesterol drives synaptic gene expression in developing neurons and reciprocal astrocytic transcriptional programs 416 samples
- GSE320162 Minimizing far-extending chromatin perturbation in genome editing preserves stem cell identity [RNA-Seq] 347 samples
- GSE317764 High-Fat Diet Causes Rapid Loss of Intestinal Group 3 Innate Lymphoid Cells Through Microbiota-Driven Inflammation 270 samples
- GSE281472 Targeting CD206+ macrophages disrupts the establishment of a key anti-tumor immune axis 245 samples
- GSE285826 SenCat: Redefining human cell senescence 232 samples
- GSE342640 Insulin resistance is associated with mammary mitochondrial dysfunction at the onset of human lactation 159 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.