GEO series

Failed reprogramming of transformed cells due to induction of apoptosis and senescence impairs tumor progression in lung cancer

GSE245949 Homo sapiens; Mus musculus Expression profiling by high throughput sequencing 24 samples Submitted 2024/12/05 Platform GPL20301Platform GPL21103

Summary

Cell reprogramming to pluripotency applied to the study of cancer has identified transformation and pluripotency as two independent and incompatible cell fates. A detailed knowledge of the relationship between transformation and reprogramming could lead to the identification of new vulnerabilities and therapeutic targets in cancer. Here, we explore this interplay and find that OSKM expression limits tumor cell growth by inducing apoptosis and senescence. We identify Oct4 and Klf4 as the main individual reprogramming factors responsible for this effect. Mechanistically, the induction of cell cycle inhibitor p21 downstream of the reprogramming factors acts as mediator of cell death and senescence. Using a variety of in vivo systems, including allografts, orthotopic transplantation and KRAS-driven lung cancer mouse models, we demonstrate that OSKM expression impairs tumor growth and reduces tumor burden

Published in

Inhibition of lung tumorigenesis by transient reprogramming in cancer cells

Pedrosa P, Zhang Z, Nuñez-Quintela V et al. · Cell death & disease 2024 · PMID 39587064 · doi:10.1038/s41419-024-07207-2

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Direct links to NCBI, no account and no request form: the whole study as GSE245949_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 24 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1030426 and SRA study SRP467617. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39587064) ↗

Study design

8 conditions, mostly in triplicate

4F_D1 ×3 4F_D3 ×3 TA_D1 ×3 TA_D3 ×3 day1_4F ×3 day1_TA ×3 day3_4F ×3 day3_TA ×3

Supports a between-group comparison across 24 samples.

8 replicated groups read from 24 sample titles; they account for 24 of them. Check it against the sample list below before relying on it.

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