← BioTransfer GEO Dataset Finder
GEO series

Reversing myeloid-derived suppressor cells mediated immunosuppression via p38α inhibition enhances immunotherapy efficacy

GSE228550 Mus musculus; Homo sapiens Expression profiling by high throughput sequencing 12 samples Submitted 2025/12/02 Platform GPL24247Platform GPL24676
Summary
By combining the tumor immunological phenotype (TIP) gene signature and high throughput sequencing based high throughput screening (HTS2), we identified that ponatinib significantly inhibits the expression of “cold” tumor associated chemokines CXCL1 and CXCL2 in cancer cells. To investigate how ponatinib regulates the expression of “cold”-tumor related genes CXCL1 and CXCL2, we conducted RNA-seq study to examine transcriptome-wide effects of ponatinib with both MDA-MB-231 cells and 4T1 cells.
Published in
Gene Signature-Based Drug Screening Reveals Ponatinib Enhances Immunotherapy Efficacy in Triple-Negative Breast Cancer by Reversing MDSC-Mediated Immunosuppressive Tumor Microenvironment
Wang Q, Li S, Wu Y et al. · Research (Washington, D.C.) 2025 · PMID 41079672 · doi:10.34133/research.0915
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE228550_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA950332 and SRA study SRP430118. Searching any of these in the dataset finder brings you back here.

Study design
3 × MDA-MB-231_DMSO vs 3 × MDA-MB-231_Ponatinib vs 3 × 4T1_DMSO vs 3 × 4T1_Ponatinib

Supports a between-group comparison across 12 samples.

4 replicated groups read from 12 sample titles; they account for 12 of them. Check it against the sample list below before relying on it.

Samples in this study
Similar datasets

Search all RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.