GEO series
Vitamin B2 Sensing by the Nuclear Receptor AhR Reprograms Hepatic Metabolism [ChIP-Seq]
GSE341320
Homo sapiens; Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
54 samples
Submitted 2026/08/01
Platform GPL30173Platform GPL34284Platform GPL34290
Summary
Nuclear receptors such as RAR/RXR and VDR directly sense vitamins A and D, but analogous sensing mechanisms for most other vitamins remain unknown. Here we identify a metabolite sensing pathway linking vitamin B2 (riboflavin) availability to an adaptive gene expression program. Riboflavin deficiency limits the FAD-dependent enzyme kynurenine 3-monooxygenase, leading to accumulation of kynurenine, an endogenous ligand of the aryl hydrocarbon receptor (AhR). Kynurenine-induced AhR nuclear translocation selectively activates poised hepatic enhancers in cooperation with AP-1 and C/EBPα, inducing glucuronidation genes and the FAD synthase gene FLAD1. This response is required to maintain intracellular FAD levels and support cell fitness under riboflavin-limiting conditions. In vivo, dietary riboflavin deficiency enhances hepatic drug clearance and reduces irinotecan toxicity. Together, these findings reveal a vitamin-sensing mechanism in which a cofactor-dependent enzyme acts as the sensor and AhR functions as the transcriptional effector that enables both flavin homeostasis and hepatic clearance capacity.
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE341320_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 54 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1501350 and SRA study SRP721051. Searching any of these in the dataset finder brings you back here.
Study design
15 conditions, mostly in duplicate
HepG2_Day12_Cont_H3K27ac_ChIP ×5
HepG2_Day12_B2_minus_H3K27ac_ChIP ×5
AhR_flox_Hepatocyte_Cont_Ad_Cont_H3K27ac_ChIP ×3
AhR_flox_Hepatocyte_B2_minus_Ad_Cont_H3K27ac_… ×3
AhR_flox_Hepatocyte_Cont_Ad_Cre_H3K27ac_ChIP ×3
HepG2_Cont_DMSO_Day12_H3K27ac_ChIP ×2
HepG2_B2_minus_DMSO_Day12_H3K27ac_ChIP ×2
HepG2_B2_minus_AhRinh_Day12_H3K27ac_ChIP ×2
+8 more
Supports a between-group comparison across 39 samples.
15 replicated groups read from the first 40 of 54 sample titles; they account for 39 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9903815 HepG2_Day12_Cont_H3K27ac_ChIP_rep1
- GSM9903816 HepG2_Day12_Cont_H3K27ac_ChIP_rep2
- GSM9903817 HepG2_Day12_Cont_H3K27ac_ChIP_rep3
- GSM9903818 HepG2_Day12_B2_minus_H3K27ac_ChIP_rep1
- GSM9903819 HepG2_Day12_B2_minus_H3K27ac_ChIP_rep2
- GSM9903820 HepG2_Day12_B2_minus_H3K27ac_ChIP_rep3
- GSM9903821 HepG2_Day12_Cont_H3K27ac_ChIP_rep4
- GSM9903822 HepG2_Day12_Cont_H3K27ac_ChIP_rep5
- GSM9903823 HepG2_Day12_B2_minus_H3K27ac_ChIP_rep4
- GSM9903824 HepG2_Day12_B2_minus_H3K27ac_ChIP_rep5
- GSM9903825 HepG2_Cont_DMSO_Day12_H3K27ac_ChIP_rep1
- GSM9903826 HepG2_Cont_DMSO_Day12_H3K27ac_ChIP_rep2
- GSM9903827 HepG2_B2_minus_DMSO_Day12_H3K27ac_ChIP_rep1
- GSM9903828 HepG2_B2_minus_DMSO_Day12_H3K27ac_ChIP_rep2
- GSM9903829 HepG2_B2_minus_AhRinh_Day12_H3K27ac_ChIP_rep1
- GSM9903830 HepG2_B2_minus_AhRinh_Day12_H3K27ac_ChIP_rep2
- GSM9903831 HepG2_Day12_Cont_AhR_ChIP_rep1
- GSM9903832 HepG2_Day12_Cont_AhR_ChIP_rep2
- GSM9903833 HepG2_Day12_B2_minus_AhR_ChIP_rep1
- GSM9903834 HepG2_Day12_B2_minus_AhR_ChIP_rep2
- GSM9903835 HepG2_Day12_Cont_AhR_ChIP_Input_rep1
- GSM9903836 HepG2_Day12_Cont_AhR_ChIP_Input_rep2
- GSM9903837 HepG2_Day12_B2_minus_AhR_CEBPa_ChIP_Input_rep1
- GSM9903838 HepG2_Day12_B2_minus_AhR_CEBPa_ChIP_Input_rep2
- GSM9903839 HepG2_Cont_Day12_CEBPa_ChIP_rep1
- GSM9903840 HepG2_Cont_Day12_CEBPa_ChIP_rep2
- GSM9903841 HepG2_Cont_Day12_CEBPa_ChIP_Input_rep1
- GSM9903842 HepG2_Cont_Day12_CEBPa_ChIP_Input_rep2
- GSM9903843 HepG2_Day12_B2_minus_CEBPa_ChIP_rep1
- GSM9903844 HepG2_Day12_B2_minus_CEBPa_ChIP_rep2
- GSM9903845 AhR_flox_Hepatocyte_Cont_Ad_Cont_H3K27ac_ChIP_rep1
- GSM9903846 AhR_flox_Hepatocyte_Cont_Ad_Cont_H3K27ac_ChIP_rep2
- GSM9903847 AhR_flox_Hepatocyte_Cont_Ad_Cont_H3K27ac_ChIP_rep3
- GSM9903848 AhR_flox_Hepatocyte_B2_minus_Ad_Cont_H3K27ac_ChIP_rep1
- GSM9903849 AhR_flox_Hepatocyte_B2_minus_Ad_Cont_H3K27ac_ChIP_rep2
- GSM9903850 AhR_flox_Hepatocyte_B2_minus_Ad_Cont_H3K27ac_ChIP_rep3
- GSM9903851 AhR_flox_Hepatocyte_Cont_Ad_Cre_H3K27ac_ChIP_rep1
- GSM9903852 AhR_flox_Hepatocyte_Cont_Ad_Cre_H3K27ac_ChIP_rep2
- GSM9903853 AhR_flox_Hepatocyte_Cont_Ad_Cre_H3K27ac_ChIP_rep3
- GSM9903854 AhR_flox_Hepatocyte_B2_minus_Ad_Cre_H3K27ac_ChIP_rep1
+ 14 more — browse all 54 samples with per-sample file links →
Similar datasets
- GSE245731 HNF4G and FOXA1 are context-specific drivers of pancreatic cancer progression [ChIP-seq] 172 samples
- GSE260742 DOT1L provides transcriptional memory through PRC1.1 antagonism [ChIP-Seq] 158 samples
- GSE287736 Sex-specific KDM6A-HNF4A-CREBH network controls lipoprotein cholesterol metabolism and atherosclerosis via epigenetic reprograming of hepatocytes 138 samples
- GSE292571 SUMOylation orchestrates a metastable heterochromatin state on a MORC3-responsive element to silence IFNB1 at a distance [ChIP-seq] 116 samples
- GSE279644 BCR::ABL1-induced enhancer reprogramming uncovers hypersensitivity of Ph+B-ALL cells to enhancer-targeting drugs [ChIP-Seq] 91 samples
- GSE229439 Tracing functional (epi)genomic imprints and their evolutionary origins in human defense antiviral cellular response (ChIP-Seq) 85 samples
- GSE274361 H3K36 Methylation - a Guardian of Epigenome Integrity [ChIP-Seq] 81 samples
- GSE292300 A core transcriptional regulatory circuitry controls super-enhancer-driven activation of LGR5 in colorectal cancer: ChIP-seq 76 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.