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Intersection of transient cell states with stable cell types in hippocampus

GSE335423 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/08/07 Platform GPL34290
Summary
The transcriptome of a brain cell encodes both its stable identity and its dynamic responses to environmental stimuli. While significant progress has been made in categorizing cell types within the brain, deciphering to what extent transcriptional identity and transcriptional state are related remains a major technical and conceptual challenge. Here, we present a single-nucleus RNA-sequencing atlas of the mouse hippocampus spanning physiological and pathological stimuli and multiple circadian phases, enabling unified analysis of activity-, circadian-, and cell-type-dependent transcriptional programs. Taxonomically assigned cell types are largely stable despite the induction of different activity states, with a notable exception in the dentate gyrus. Activity and circadian rhythm each drive robust, largely nonoverlapping transcriptional responses, with convergent regulation on genes involved in specific pathways, including endocannabinoid signaling, excitability, and chromatin remodeling. These results underscore the necessity of integrating cell-type taxonomy with transcriptional state to capture how diverse cell types respond to experience.
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Direct links to NCBI, no account and no request form: the whole study as GSE335423_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1478526 and SRA study SRP709670. Searching any of these in the dataset finder brings you back here.

Study design
A cohort of 8 individual samples

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Sample titles run Sublibrary 1 … Sublibrary 8 and carry no group information.

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