GEO series
Reversible epiblast regionalisation determines differentiation potential of human PSCs [ATAC-seq]
GSE334112
Homo sapiens
Genome binding/occupancy profiling by high throughput sequencing
38 samples
Submitted 2026/06/30
Platform GPL34281
Summary
Although the epiblast in the embryo has the capacity to generate all tissues of the body, its in vitro counterparts often exhibit differentiation biases, posing significant challenges for both basic research and translational applications involving pluripotent stem cells (PSCs). The origins of these biases remain incompletely understood. In this study, we identify regional epiblast identity as the hallmark of differentiation competency through fluctuations in repressive and activating histone posttranslational modifications. We present a novel approach to overcome this bias using a chemical chromatin restoration (CHR) treatment. This method restores transcriptional programs, chromatin accessibility, histone modification profiles, and differentiation potential, effectively recapitulating the competent anterior epiblast-like state. Furthermore, we propose that a high bivalency state is a defining feature of the anterior human epiblast and is a characteristic of unbiased PSCs. We suggest that fluctuations in histone modification marks drive epiblast regionalization, ultimately shaping cellular responses to differentiation cues.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE334112_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 38 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1473740 and SRA study SRP706153. Searching any of these in the dataset finder brings you back here.
Study design
15 conditions, mostly in duplicate
burb1_untreated ×4
fiaj1_untreated ×4
hehd1_untreated ×4
sojd3_untreated ×4
H1_untreated ×2
H9_untreated ×2
HUES8_untreated ×2
kolf2_untreated ×2
+7 more
Supports a between-group comparison across 38 samples.
15 replicated groups read from 38 sample titles; they account for 38 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9781265 burb1_untreated_rep1
- GSM9781266 burb1_untreated_rep2
- GSM9781267 fiaj1_untreated_rep1
- GSM9781268 fiaj1_untreated_rep2
- GSM9781269 H1_untreated_rep1
- GSM9781270 H1_untreated_rep2
- GSM9781271 H9_untreated_rep1
- GSM9781272 H9_untreated_rep2
- GSM9781273 hehd1_untreated_rep1
- GSM9781274 hehd1_untreated_rep2
- GSM9781275 HUES8_untreated_rep1
- GSM9781276 HUES8_untreated_rep2
- GSM9781277 kolf2_untreated_rep1
- GSM9781278 kolf2_untreated_rep2
- GSM9781279 sojd3_untreated_rep1
- GSM9781280 sojd3_untreated_rep2
- GSM9781281 burb1_untreated_rep3
- GSM9781282 burb1_untreated_rep4
- GSM9781283 burb1_CHR_rep1
- GSM9781284 burb1_CHR_rep2
- GSM9781285 fiaj1_untreated_rep3
- GSM9781286 fiaj1_untreated_rep4
- GSM9781287 fiaj1_CHR_rep1
- GSM9781288 fiaj1_CHR_rep2
- GSM9781289 hehd1_untreated_rep3
- GSM9781290 hehd1_untreated_rep4
- GSM9781291 hehd1_CHR_rep1
- GSM9781292 hehd1_CHR_rep2
- GSM9781293 sojd3_untreated_rep3
- GSM9781294 sojd3_untreated_rep4
- GSM9781295 sojd3_CHR_rep1
- GSM9781296 sojd3_CHR_rep2
- GSM9781297 cuhk2_untreated_rep1
- GSM9781298 cuhk2_untreated_rep2
- GSM9781299 deyz2_untreated_rep1
- GSM9781300 deyz2_untreated_rep2
- GSM9781301 feec2_untreated_rep1
- GSM9781302 feec2_untreated_rep2
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