GEO series
BATF3 regulates chromatin accessibility during CD8+ T lymphocyte differentiation
GSE325835
Homo sapiens
Genome binding/occupancy profiling by high throughput sequencing
36 samples
Submitted 2026/04/10
Platform GPL34284
Summary
CD8+ T lymphocytes differentiate from effector to memory cells following antigen clearance, with prolonged IL-2 production characterizing functional cytotoxic T lymphocytes (CTLs). To identify transcription factors associated with sustained IL-2 production, we compared influenza virus-specific and cytomegalovirus-specific CTLs, identifying Basic Leucine Zipper ATF-Like Transcription Factor 3 (BATF3) as a key candidate gene. BATF3 overexpression significantly enhanced cell proliferation in both virus-specific CTLs and CD19 chimeric antigen receptor T (CAR-T) cells while reducing cytokine production. ATAC-seq analysis revealed that BATF3 overexpression dynamically regulates chromatin accessibility, affecting diverse cellular processes including cytoskeletal organization, metabolic pathways, and cellular survival signaling. BATF3 specifically facilitated the critical transition from effector to memory phase. These findings establish BATF3 as a master regulator of CD8+ T cell fate determination through dynamic chromatin remodeling mechanisms.
Published in
Umemura K, Kojima Y, Julamanee J et al.
· Life science alliance 2026
· PMID 41974573
· doi:10.26508/lsa.202503550
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE325835_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 36 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1442034 and SRA study SRP686235. Searching any of these in the dataset finder brings you back here.
Study design
18 conditions, mostly in duplicate
Untransduced CD8+ T cell Day 0, Donor F ×2
Untransduced CD8+ T cell Day 7, Donor F ×2
Untransduced CD8+ T cell Day 14, Donor F ×2
BATF CD8+ T cell Day 0, Donor F ×2
BATF CD8+ T cell Day 7, Donor F ×2
BATF CD8+ T cell Day 14, Donor F ×2
BATF3 CD8+ T cell Day 0, Donor F ×2
BATF3 CD8+ T cell Day 7, Donor F ×2
+10 more
Supports a between-group comparison across 36 samples.
18 replicated groups read from 36 sample titles; they account for 36 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9614279 Untransduced CD8+ T cell Day 0, Donor F1
- GSM9614280 Untransduced CD8+ T cell Day 7, Donor F1
- GSM9614281 Untransduced CD8+ T cell Day 14, Donor F1
- GSM9614282 BATF CD8+ T cell Day 0, Donor F1
- GSM9614283 BATF CD8+ T cell Day 7, Donor F1
- GSM9614284 BATF CD8+ T cell Day 14, Donor F1
- GSM9614285 BATF3 CD8+ T cell Day 0, Donor F1
- GSM9614286 BATF3 CD8+ T cell Day 7, Donor F1
- GSM9614287 BATF3 CD8+ T cell Day 14, Donor F1
- GSM9614288 Untransduced CD8+ T cell Day 0, Donor U1
- GSM9614289 Untransduced CD8+ T cell Day 7, Donor U1
- GSM9614290 Untransduced CD8+ T cell Day 14, Donor U1
- GSM9614291 BATF CD8+ T cell Day 0, Donor U1
- GSM9614292 BATF CD8+ T cell Day 7, Donor U1
- GSM9614293 BATF CD8+ T cell Day 14, Donor U1
- GSM9614294 BATF3 CD8+ T cell Day 0, Donor U1
- GSM9614295 BATF3 CD8+ T cell Day 7, Donor U1
- GSM9614296 BATF3 CD8+ T cell Day 14, Donor U1
- GSM9614297 Untransduced CD8+ T cell Day 0, Donor F2
- GSM9614298 Untransduced CD8+ T cell Day 7, Donor F2
- GSM9614299 Untransduced CD8+ T cell Day 14, Donor F2
- GSM9614300 BATF CD8+ T cell Day 0, Donor F2
- GSM9614301 BATF CD8+ T cell Day 7, Donor F2
- GSM9614302 BATF CD8+ T cell Day 14, Donor F2
- GSM9614303 BATF3 CD8+ T cell Day 0, Donor F2
- GSM9614304 BATF3 CD8+ T cell Day 7, Donor F2
- GSM9614305 BATF3 CD8+ T cell Day 14, Donor F2
- GSM9614306 Untransduced CD8+ T cell Day 0, Donor U2
- GSM9614307 Untransduced CD8+ T cell Day 7, Donor U2
- GSM9614308 Untransduced CD8+ T cell Day 14, Donor U2
- GSM9614309 BATF CD8+ T cell Day 0, Donor U2
- GSM9614310 BATF CD8+ T cell Day 7, Donor U2
- GSM9614311 BATF CD8+ T cell Day 14, Donor U2
- GSM9614312 BATF3 CD8+ T cell Day 0, Donor U2
- GSM9614313 BATF3 CD8+ T cell Day 7, Donor U2
- GSM9614314 BATF3 CD8+ T cell Day 14, Donor U2
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