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Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling [CUT&RUN]

GSE278910 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 30 samples Submitted 2024/10/13 Platform GPL24676
Summary
MultiPerturb-seq is a high-throughput CRISPR screening platform with joint single nucleus chromatin accessibility, transcriptome, and guide RNA capture. It uses combinatorial indexing combined with droplet microfluidics to scale throughput and integrate all three modalities. We apply MultiPerturb-seq to identify key genes whose loss can trigger differentiation in a rare pediatric cancer, atypical teratoid/rhabdoid tumor (AT/RT), which is driven by loss of the SWI/SNF chromatin remodeling subunit SMARCB1.
Published in
Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling
Yan RE, Corman A, Katgara L et al. · Nature biotechnology 2025 · PMID 39572737 · doi:10.1038/s41587-024-02475-x
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE278910_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 30 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1169554 and SRA study SRP536880. Searching any of these in the dataset finder brings you back here.

Study design
6 conditions, mostly with about 5 replicates each
NT_H2AZ ×5 NT_H3K4me3 ×5 NT_IgG ×5 ZNHIT1_H2AZ ×5 ZNHIT1_H3K4me3 ×5 ZNHIT1_IgG ×5

Supports a between-group comparison across 30 samples.

6 replicated groups read from 30 sample titles; they account for 30 of them. Check it against the sample list below before relying on it.

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