GEO series
Epigenomic profiling of H3K27ac in mouse brain regions under morphine and LPS exposure
GSE332794
Mus musculus
Genome binding/occupancy profiling by high throughput sequencing
206 samples
Submitted 2026/06/17
Platform GPL34290
Summary
We profiled genome-wide H3K27ac chromatin modifications across four brain regions (hippocampus, prefrontal cortex, striatum, and hypothalamus) in male and female C57BL/6NTac mice using a perinatal morphine exposure model of Neonatal Opioid Withdrawal Syndrome (NOWS). Mice received morphine or saline perinatally, then were challenged with LPS or saline in adulthood, yielding four groups (SAL-SAL, SAL-LPS, MOR-SAL, MOR-LPS). NeuN+ neuronal nuclei (~10,000 per replicate) were isolated by FACS and profiled by MOWChIP-seq (Illumina NovaSeq X Plus, paired-end 150 nt). H3K27ac ChIP-seq identified differentially acetylated enhancers and promoters enriched in immune and metabolic pathways, with the hypothalamus exhibiting the most pronounced chromatin remodeling.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE332794_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 206 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1469104 and SRA study SRP702673. Searching any of these in the dataset finder brings you back here.
Study design
31 conditions, each sampled once — no replicated groups
H3K27ac ChIP-seq hippocampus, SAL-LPS, female… ×3
H3K27ac ChIP-seq hippocampus, MOR-LPS, male, … ×2
H3K27ac ChIP-seq hippocampus, MOR-SAL, female… ×2
H3K27ac ChIP-seq hippocampus, MOR-SAL, male, … ×2
H3K27ac ChIP-seq hippocampus, SAL-LPS, female… ×2
H3K27ac ChIP-seq hippocampus, SAL-LPS, female… ×2
H3K27ac ChIP-seq hippocampus, SAL-LPS, male, … ×2
H3K27ac ChIP-seq hippocampus, SAL-LPS, male, … ×2
Read from the first 40 of 206 sample titles: 31 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9753374 H3K27ac ChIP-seq hippocampus, MOR-LPS, female, replicate 12-0
- GSM9753375 Input control hippocampus, MOR-LPS, female
- GSM9753376 H3K27ac ChIP-seq hippocampus, MOR-LPS, female, replicate 15-3
- GSM9753377 H3K27ac ChIP-seq hippocampus, MOR-LPS, female, replicate 16-4
- GSM9753378 H3K27ac ChIP-seq hippocampus, MOR-LPS, female, replicate 18-1
- GSM9753379 H3K27ac ChIP-seq hippocampus, MOR-LPS, male, replicate 13-2
- GSM9753380 Input control hippocampus, MOR-LPS, male
- GSM9753381 H3K27ac ChIP-seq hippocampus, MOR-LPS, male, replicate 14-2
- GSM9753382 H3K27ac ChIP-seq hippocampus, MOR-LPS, male, replicate 17-1
- GSM9753383 H3K27ac ChIP-seq hippocampus, MOR-LPS, male, replicate 6-0
- GSM9753384 H3K27ac ChIP-seq hippocampus, MOR-LPS, male, replicate 6-4
- GSM9753385 H3K27ac ChIP-seq hippocampus, MOR-LPS, male, replicate 8-3
- GSM9753386 H3K27ac ChIP-seq hippocampus, MOR-SAL, female, replicate 12-1
- GSM9753387 Input control hippocampus, MOR-SAL, female
- GSM9753388 H3K27ac ChIP-seq hippocampus, MOR-SAL, female, replicate 15-0
- GSM9753389 H3K27ac ChIP-seq hippocampus, MOR-SAL, female, replicate 16-1
- GSM9753390 H3K27ac ChIP-seq hippocampus, MOR-SAL, female, replicate 7-2
- GSM9753391 H3K27ac ChIP-seq hippocampus, MOR-SAL, female, replicate 7-3
- GSM9753392 H3K27ac ChIP-seq hippocampus, MOR-SAL, male, replicate 13-1
- GSM9753393 Input control hippocampus, MOR-SAL, male
- GSM9753394 H3K27ac ChIP-seq hippocampus, MOR-SAL, male, replicate 14-1
- GSM9753395 H3K27ac ChIP-seq hippocampus, MOR-SAL, male, replicate 17-4
- GSM9753396 H3K27ac ChIP-seq hippocampus, MOR-SAL, male, replicate 6-1
- GSM9753397 H3K27ac ChIP-seq hippocampus, MOR-SAL, male, replicate 8-0
- GSM9753398 H3K27ac ChIP-seq hippocampus, MOR-SAL, male, replicate 8-2
- GSM9753399 H3K27ac ChIP-seq hippocampus, SAL-LPS, female, replicate 10-0
- GSM9753400 Input control hippocampus, SAL-LPS, female
- GSM9753401 H3K27ac ChIP-seq hippocampus, SAL-LPS, female, replicate 10-2
- GSM9753402 H3K27ac ChIP-seq hippocampus, SAL-LPS, female, replicate 10-4
- GSM9753403 H3K27ac ChIP-seq hippocampus, SAL-LPS, female, replicate 2-0
- GSM9753404 H3K27ac ChIP-seq hippocampus, SAL-LPS, female, replicate 2-2
- GSM9753405 H3K27ac ChIP-seq hippocampus, SAL-LPS, female, replicate 4-0
- GSM9753406 H3K27ac ChIP-seq hippocampus, SAL-LPS, female, replicate 4-2
- GSM9753407 H3K27ac ChIP-seq hippocampus, SAL-LPS, male, replicate 1-0
- GSM9753408 Input control hippocampus, SAL-LPS, male
- GSM9753409 H3K27ac ChIP-seq hippocampus, SAL-LPS, male, replicate 1-2
- GSM9753410 H3K27ac ChIP-seq hippocampus, SAL-LPS, male, replicate 11-0
- GSM9753411 H3K27ac ChIP-seq hippocampus, SAL-LPS, male, replicate 3-3
- GSM9753412 H3K27ac ChIP-seq hippocampus, SAL-LPS, male, replicate 5-0
- GSM9753413 H3K27ac ChIP-seq hippocampus, SAL-LPS, male, replicate 5-2
+ 166 more — browse all 206 samples with per-sample file links →
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