GEO series
Hepatic HuR protects against atherosclerosis through bile acid production
GSE326269
Homo sapiens; Mus musculus
Expression profiling by high throughput sequencing
38 samples
Submitted 2026/05/31
Platform GPL24247Platform GPL24676
Summary
Hepatocyte-specific HuR knockout mice exhibit a lean MAFLD (metabolic-associated fatty liver disease)-like phenotype in response to high-fat diet. However, if this phenotype further impacts the development of atherosclerosis (AS) remains to be studied. Here, we report that HuR represses the production of chenodeoxycholic acid (CDCA) and taurocholic acid (TCA) in the liver through repressing the expression of CYP7A1, the rate-limiting enzyme for the central pathway of BA production. HuR destabilizes Cyp7a1 mRNA by associating with its 3’UTR. In response to high-cholesterol diet (HCD), hepatic HuR increases the expression of CYP7A1 in the liver, thereby increasing the levels of CDCA and TCA in the liver and serum, activating HuR-PSPF (platelet-secreted pro-inflammatory factors) regulatory process, and aggravating HCD-induced AS in APOE (-/-) mice. Administration of TCA and CDCA activates HuR-PSPF regulatory process and at the same time aggravates HCD-induced AS. Platelet HuR ablation mitigates the effect of hepatic HuR ablation in aggravating HCD-induced AS. In the liver tissues from patients with lean MAFLD, the reduction of HuR expression, the induction of CYP7A1 expression and the activation of HuR-PFPF regulatory process, are concurrently observed. Our findings suggest an important role of hepatic HuR-CYP7A1 regulatory process in the development of AS in lean MAFLD-like lipid dysregulation.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE326269_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 38 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1445032 and SRA study SRP687630. Searching any of these in the dataset finder brings you back here.
Study design
12 × HM-CT vs 12 × HM-LM vs 6 × PH-WT vs 6 × PH-cKO
Supports a between-group comparison across 36 samples.
4 replicated groups read from 38 sample titles; they account for 36 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9626457 PH-WT1
- GSM9626458 PH-WT2
- GSM9626459 PH-WT3
- GSM9626460 PH-WT4
- GSM9626461 PH-WT5
- GSM9626462 PH-WT6
- GSM9626463 PH-cKO1
- GSM9626464 PH-cKO2
- GSM9626465 PH-cKO3
- GSM9626466 PH-cKO4
- GSM9626467 PH-cKO5
- GSM9626468 PH-cKO6
- GSM9626469 PLT-WT
- GSM9626470 PLT-cKO
- GSM9626471 HM-CT1
- GSM9626472 HM-CT2
- GSM9626473 HM-CT3
- GSM9626474 HM-CT4
- GSM9626475 HM-CT5
- GSM9626476 HM-CT6
- GSM9626477 HM-CT7
- GSM9626478 HM-CT8
- GSM9626479 HM-CT9
- GSM9626480 HM-CT10
- GSM9626481 HM-CT11
- GSM9626482 HM-CT12
- GSM9626483 HM-LM1
- GSM9626484 HM-LM2
- GSM9626485 HM-LM3
- GSM9626486 HM-LM4
- GSM9626496 HM-LM5
- GSM9626497 HM-LM6
- GSM9626498 HM-LM7
- GSM9626499 HM-LM8
- GSM9626500 HM-LM9
- GSM9626501 HM-LM10
- GSM9626502 HM-LM11
- GSM9626503 HM-LM12
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