GEO series
Viral entry defines the hepatitis E virus species barrier in murine hepatocytes
GSE319021
Mus musculus; Homo sapiens
Expression profiling by high throughput sequencing
60 samples
Submitted 2026/08/07
Platform GPL34290Platform GPL32159Platform GPL30882
Summary
The Paslahepevirus balayani hepatitis E virus (HEV) and the distantly related Rocahepevirus ratti rat HEV pose a risk for zoonotic transmission to humans. However, the molecular determinants for HEV transmission between species remain unknown. Despite the broad host range including its ability to infect certain rodent species, infections of animals of the genus Mus within the subfamily Murinae are rarely documented. To dissect the molecular mechanisms underlying species barriers to HEV infection, this study aimed to investigate the virus replication cycle and immune-related determinants responsible for restricted HEV infection in murine hepatocytes. Murine hepatic cell lines supported moderate levels of zoonotic HEV replication and infectious virion production upon transfection of in vitro transcribed viral RNA. Notably, viral replication was not restricted by innate immune responses or presence of dominant restriction factors but was limited by absence of host-specific dependency factors. While successful attachment to murine hepatic cells was detected, mechanisms of viral entry differed between human and murine hepatocytes, correlating with murine cell lines and primary murine hepatocytes being refractory to HEV infection. In summary, the murine barrier to HEV infection is defined at the viral entry stage, specifically by a block post attachment and before viral replication is initiated. These findings shed new light on the fundamental role of viral entry mechanisms in defining HEV species tropism.
Published in
Frericks N, Pinto Veiga O, Sirkinti L et al.
· Emerging microbes & infections 2026
· PMID 42506980
· doi:10.1080/22221751.2026.2706321
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE319021_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 60 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1421980 and SRA study SRP676087. Searching any of these in the dataset finder brings you back here.
Study design
40 conditions, each sampled once — no replicated groups
Read from the first 40 of 60 sample titles: 40 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9508022 NF74, HepG2, tRNA, Mock
- GSM9508023 NF74, HepG2, tRNA, IFN
- GSM9508024 NF74, HepG2, p6, Mock
- GSM9508025 NF74, HepG2, p6, IFN
- GSM9508026 NF81, HepG2, tRNA, Mock
- GSM9508027 NF81, HepG2, tRNA, IFN
- GSM9508028 NF81, HepG2, p6, Mock
- GSM9508029 NF81, HepG2, p6, IFN
- GSM9508030 NF84, HepG2, tRNA, Mock
- GSM9508031 NF84, HepG2, tRNA, IFN
- GSM9508032 NF84, HepG2, p6, Mock
- GSM9508033 NF84, HepG2, p6, IFN
- GSM9508034 NF74, MLT, tRNA, Mock
- GSM9508035 NF74, MLT, tRNA, IFN
- GSM9508036 NF74, MLT, p6, Mock
- GSM9508037 NF74, MLT, p6, IFN
- GSM9508038 NF74, 56D, tRNA, Mock
- GSM9508039 NF74, 56D, tRNA, IFN
- GSM9508040 NF74, 56D, p6, Mock
- GSM9508041 NF74, 56D, p6, IFN
- GSM9508042 NF81, MLT, tRNA, Mock
- GSM9508043 NF81, MLT, tRNA, IFN
- GSM9508044 NF81, MLT, p6, Mock
- GSM9508045 NF81, MLT, p6, IFN
- GSM9508046 NF81, 56D, tRNA, Mock
- GSM9508047 NF81, 56D, tRNA, IFN
- GSM9508048 NF81, 56D, p6, Mock
- GSM9508049 NF81, 56D, p6, IFN
- GSM9508050 NF84, MLT, tRNA, Mock
- GSM9508051 NF84, MLT, tRNA, IFN
- GSM9508052 NF84, MLT, p6, Mock
- GSM9508053 NF84, MLT, p6, IFN
- GSM9508054 NF84, 56D, tRNA, Mock
- GSM9508055 NF84, 56D, tRNA, IFN
- GSM9508056 NF84, 56D, p6, Mock
- GSM9508057 NF84, 56D, p6, IFN
- GSM9508058 Maus MH210923, CM, 6 hpi
- GSM9508059 Maus MH210923, p6, 6 hpi
- GSM9508060 Maus MH211103, CM, 6 hpi
- GSM9508061 Maus MH211103, p6, 6 hpi
+ 20 more — browse all 60 samples with per-sample file links →
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