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Effect of Dox-induced Cas13 expression in stable cell lines on the mouse transcriptome and co-cultured human PSCs

GSE307132 Mus musculus; Homo sapiens Expression profiling by high throughput sequencing 21 samples Submitted 2025/12/29 Platform GPL34284Platform GPL34290
Summary
In the mouse EpiSCs-iCas13 stable cell line, addition of doxycycline induces Cas13 expression, which further triggers collateral effects and slows down cell proliferation. In co-culture of mouse and human cells, under normal conditions, mouse EpiSCs hold a proliferative advantage, whereas human PSCs are at a disadvantage and show almost no growth. Upon doxycycline treatment in the co-culture, Cas13 expression in mouse cells triggers collateral effects that reduce their proliferation, thereby providing a relative advantage for human PSCs. As a result, the proliferation of human PSCs is markedly enhanced, showing a growth pattern more comparable to that observed in separate cultures of human PSCs.
Published in
Mitigating xenogeneic barriers to chimerism through Cas13-induced host attenuation
He B, Lv S, Li L et al. · Developmental cell 2026 · PMID 41616766 · doi:10.1016/j.devcel.2026.01.001
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Direct links to NCBI, no account and no request form: the whole study as GSE307132_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 21 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1314640 and SRA study SRP617687. Searching any of these in the dataset finder brings you back here.

Study design
7 conditions, mostly in triplicate
cc_dox_mouse ×3 cc_dox free_mouse ×3 sc_dox_mouse ×3 sc_dox free_mouse ×3 cc_dox_human ×3 cc_dox free_human ×3 sc_dox free_human ×3

Supports a between-group comparison across 21 samples.

7 replicated groups read from 21 sample titles; they account for 21 of them. Check it against the sample list below before relying on it.

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