← BioTransfer GEO Dataset Finder
GEO series

Hepatic leukemia factor directs tissue residency of proinflammatory CD4+ T cells

GSE295436 Homo sapiens; Mus musculus Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 22 samples Submitted 2025/07/04 Platform GPL24676Platform GPL24247
Summary
CD4+ tissue-resident memory T cells (TRM) contribute to both host defense and pathogenesis of chronic inflammatory diseases. However, the molecular mechanisms that direct tissue residency and functional heterogeneity of CD4+ TRM remain unknown. We herein show that the transcription factor, hepatic leukemia factor (HLF), directs the tissue residency program and functionality of CD4+ TRM. HLF simultaneously upregulates tissue retention receptors and downregulates tissue egress receptors via changes in chromatin accessibility, and drives proinflammatory CD4+ TRM by inducing Bhlhe40. Genetic deletion of Hlf inhibits CD4+ TRM generation, consequently ameliorating airway tissue inflammation in vivo. In humans, HLF-positive CD4+ TRM from inflamed airway tissue have a tissue residency signature and express inflammatory cytokines. HLF is therefore a central regulator of proinflammatory CD4+ TRM development and function.
Published in
Hepatic leukemia factor directs tissue residency of proinflammatory memory CD4(+) T cells
Kiuchi M, Nemoto M, Yagyu H et al. · Science (New York, N.Y.) 2025 · PMID 41379973 · doi:10.1126/science.adp0714
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE295436_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 22 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1254670 and SRA study SRP580812. Searching any of these in the dataset finder brings you back here.

Study design
5 × ECRS_PBMC vs 5 × ECRS_Polyp vs 2 × scRNA_7w_Asp_ivnega vs 2 × scRNA_7w_Asp_ivposi

Supports a between-group comparison across 14 samples.

4 replicated groups read from 22 sample titles; they account for 14 of them. Check it against the sample list below before relying on it.

Samples in this study
Similar datasets

Search all RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.