GEO series
Inflammation awakens dormant cancer cells by modulating epithelial-mesenchymal phenotypic state
GSE280817
Mus musculus; Homo sapiens
Expression profiling by high throughput sequencing
20 samples
Submitted 2025/08/19
Platform GPL22245Platform GPL24676Platform GPL16791
Summary
The awakening of dormant disseminated cancer cells appears to be responsible for the clinical relapses of patients whose primary tumors have been successfully cured months and even years earlier. In the present study, we demonstrate that dormant breast cancer cells lodged in the lungs reside in a highly mesenchymal, non-proliferative phenotypic state. The awakening of these cells is not triggered by a cancer cell-autonomous process. Instead, lung inflammation induced by the chemotherapeutic agent bleomycin effectively awakens dormant cancer cells, providing useful models for studying metastatic awakening. Mechanistically, the awakened cells shift from a highly mesenchymal to a quasi-mesenchymal phenotypic state in which they acquire tumorigenicity and proliferative ability. Once awakened, these cells can stably reside in this quasi-mesenchymal state and maintain their tumor-initiating ability, doing so without ongoing heterotypic signaling from the lung microenvironment. EGFR ligands released by the cells of the injured tissue microenvironment, including notably M2 type macrophages, promote dormant cancer cells to move toward this quasi-mesenchymal state, a transition that is critical for the awakening process. An understanding of the mechanisms of metastatic awakening may lead in the future to treatment strategies designed to prevent such awakening and resulting metastatic relapse.
Published in
Zhang J, Zhang J, Han L et al.
· Proceedings of the National Academy of Sciences of the United States of America 2025
· PMID 40901881
· doi:10.1073/pnas.2515009122
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE280817_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 20 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1180601 and SRA study SRP506264. Searching any of these in the dataset finder brings you back here.
Study design
5 conditions, mostly in duplicate
Sum159 ITGB4 low clone 1 biol ×3
Sum159 ITGB4 low clone 1 bleomycin treated bi… ×3
Day3 control ×2
Day3 Bleomycin ×2
Sum159 ITGB4 high cells biol ×2
Day7 control ×1
Day7 Bleomycin ×1
Sum159 ITGB4 high cells ×1
+5 more
Supports a case/control comparison: 6 samples read as cases, 5 as controls.
5 replicated groups read from 20 sample titles; they account for 12 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM8257375 Day3 control 1
- GSM8257376 Day3 Bleomycin 1
- GSM8257377 Day7 control
- GSM8257378 Day7 Bleomycin
- GSM8257379 Day3 Bleomycin 2
- GSM8257380 Day3 control 2
- GSM8257381 Sum159 ITGB4 high cells
- GSM8257382 Sum159 ITGB low clone 1 beomycin treated
- GSM8257383 Sum159 ITGB low clone 1 control
- GSM8257384 Sum159 ITGB low clone 1 induced by LM2
- GSM8257385 Sum159 ITGB low clone 3 control
- GSM8257386 Sum159 ITGB low clone 3 induced by LM2
- GSM8797601 Sum159 ITGB4 high cells biol rep 1
- GSM8797602 Sum159 ITGB4 high cells biol rep 2
- GSM8797603 Sum159 ITGB4 low clone 1 biol rep 1
- GSM8797604 Sum159 ITGB4 low clone 1 biol rep 2
- GSM8797605 Sum159 ITGB4 low clone 1 biol rep 3
- GSM8797606 Sum159 ITGB4 low clone 1 bleomycin treated biol rep 1
- GSM8797607 Sum159 ITGB4 low clone 1 bleomycin treated biol rep 2
- GSM8797608 Sum159 ITGB4 low clone 1 bleomycin treated biol rep 3
Similar datasets
- GSE289193 Histone H4 lysine 20 tri-methylation safeguards breast cancer lineage fidelity and unfolded protein response 160 samples
- GSE296402 Interleukin-23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity 66 samples
- GSE331030 Lentiviral single-cell MPRA of synthetic enhancers reveals motif affinity-based encoding of cell state specificity [sc-lentiMPRA 2] 66 samples
- GSE304029 BRD2 Bromodomain-Mediated Regulation of Cell State Plasticity Modulates Therapy Response in Glioblastoma 56 samples
- GSE294887 Individual RNA-seq for different prostate cancer cells, tumors treated by LGK974, ENZ, EPZ6438, EED226 or by CTNNB1 gene knockdown and overexpression, and BRN2 gene knockdown 36 samples
- GSE328222 Acute rapamycin treatment reveals novel mechanisms of dysfunction in a maternal inflammation mouse model 32 samples
- GSE303043 UHRF1 drives subtype-independent aggressiveness and immune evasion in small cell lung cancer through GATA2-ST6GALNAC5 and PRC2 interactions 30 samples
- GSE304108 Drug Screening in 3D Microtumors Reveals DDR1/2-MAPK12-GLI1 as a Vulnerability in Cancer-Associated Fibroblasts 29 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.