GEO series
Characterize the effects of L. asaccharolyticus on autistic-like symptoms
GSE324679
Homo sapiens; Mus musculus
Expression profiling by high throughput sequencing
51 samples
Submitted 2026/04/10
Platform GPL34284Platform GPL34290
Summary
Accumulating evidence has revealed a strong link between the gut microbial community and ASD symptoms mediated by the gut–brain axis; however, ASD-specific microbial markers and the underlying mechanisms for targeted interventions remain unclear. Based on our previous identification of Lawsonibacter asaccharolyticus loss as a candidate ASD marker, we performed bacterial intervention in the BTBR ASD mouse model (C57 mice as healthy controls). Bulk RNA-seq was conducted on brain cortex and colon sections. In vitro validation: SH-SY5Y neuronal cells were treated for 24 h with bacterial filtrate, its derivative metabolite oleoylethanolamide (OEA), or butyrate (BA); untreated cells served as controls. All samples were subsequently analyzed by bulk RNA-seq.
This dataset
Download
Direct links to NCBI, no account and no request form: the whole study as GSE324679_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 51 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1436552 and SRA study SRP683198. Searching any of these in the dataset finder brings you back here.
Study design
7 conditions, mostly in triplicate
colon in BTBR mice with bacterial treatment, … ×10
colon in BTBR mice with vehicle treatment, re… ×9
SH-SY5Y human neuroblastoma cell with no trea… ×6
colon in C57 mice healthy control, replication ×5
SH-SY5Y human neuroblastoma cell treated with… ×3
SH-SY5Y human neuroblastoma cell treated with… ×3
SH-SY5Y human neuroblastoma cell treated with… ×3
brain cortex in C57 mice healthy control, rep… ×1
Supports a case/control comparison: 9 samples read as cases, 15 as controls.
7 replicated groups read from the first 40 of 51 sample titles; they account for 39 of them. Check it against the sample list below before relying on it.
Samples in this study
0 selected
- GSM9582857 SH-SY5Y human neuroblastoma cell with no treatment after 24 hours, replication 1
- GSM9582858 SH-SY5Y human neuroblastoma cell with no treatment after 24 hours, replication 2
- GSM9582859 SH-SY5Y human neuroblastoma cell with no treatment after 24 hours, replication 3
- GSM9582860 SH-SY5Y human neuroblastoma cell treated with bacterial supernatant after 24 hours, replication 1
- GSM9582861 SH-SY5Y human neuroblastoma cell treated with bacterial supernatant after 24 hours, replication 2
- GSM9582862 SH-SY5Y human neuroblastoma cell treated with bacterial supernatant after 24 hours, replication 3
- GSM9582863 SH-SY5Y human neuroblastoma cell with no treatment after 24 hours, replication 4
- GSM9582864 SH-SY5Y human neuroblastoma cell with no treatment after 24 hours, replication 5
- GSM9582865 SH-SY5Y human neuroblastoma cell with no treatment after 24 hours, replication 6
- GSM9582866 SH-SY5Y human neuroblastoma cell treated with butyrate after 24 hours, replication 1
- GSM9582867 SH-SY5Y human neuroblastoma cell treated with butyrate after 24 hours, replication 2
- GSM9582868 SH-SY5Y human neuroblastoma cell treated with butyrate after 24 hours, replication 3
- GSM9582869 SH-SY5Y human neuroblastoma cell treated with oleoylethanolamide after 24 hours, replication 1
- GSM9582870 SH-SY5Y human neuroblastoma cell treated with oleoylethanolamide after 24 hours, replication 2
- GSM9582871 SH-SY5Y human neuroblastoma cell treated with oleoylethanolamide after 24 hours, replication 3
- GSM9582872 colon in C57 mice healthy control, replication 1
- GSM9582873 colon in C57 mice healthy control, replication 2
- GSM9582874 colon in C57 mice healthy control, replication 3
- GSM9582875 colon in C57 mice healthy control, replication 4
- GSM9582876 colon in C57 mice healthy control, replication 5
- GSM9582877 colon in BTBR mice with vehicle treatment, replication 1
- GSM9582878 colon in BTBR mice with vehicle treatment, replication 2
- GSM9582879 colon in BTBR mice with vehicle treatment, replication 3
- GSM9582880 colon in BTBR mice with vehicle treatment, replication 4
- GSM9582881 colon in BTBR mice with vehicle treatment, replication 5
- GSM9582882 colon in BTBR mice with vehicle treatment, replication 6
- GSM9582883 colon in BTBR mice with vehicle treatment, replication 7
- GSM9582884 colon in BTBR mice with vehicle treatment, replication 8
- GSM9582885 colon in BTBR mice with vehicle treatment, replication 9
- GSM9582886 colon in BTBR mice with bacterial treatment, replication 1
- GSM9582887 colon in BTBR mice with bacterial treatment, replication 2
- GSM9582888 colon in BTBR mice with bacterial treatment, replication 3
- GSM9582889 colon in BTBR mice with bacterial treatment, replication 4
- GSM9582890 colon in BTBR mice with bacterial treatment, replication 5
- GSM9582891 colon in BTBR mice with bacterial treatment, replication 6
- GSM9582892 colon in BTBR mice with bacterial treatment, replication 7
- GSM9582893 colon in BTBR mice with bacterial treatment, replication 8
- GSM9582894 colon in BTBR mice with bacterial treatment, replication 9
- GSM9582895 colon in BTBR mice with bacterial treatment, replication 10
- GSM9582896 brain cortex in C57 mice healthy control, replication 1
+ 11 more — browse all 51 samples with per-sample file links →
Similar datasets
- GSE270229 Inhibition of miR-10b treats metastatic breast cancer by targeting stem cell-like properties 18 samples
- GSE276724 Denfivontinib activates effector T-cells through NLRP3-inflammasome, yielding potent anticancer effects by combination with pembrolizumab 15 samples
- GSE289420 Astrocyte-derived cholesterol drives synaptic gene expression in developing neurons and reciprocal astrocytic transcriptional programs 416 samples
- GSE320162 Minimizing far-extending chromatin perturbation in genome editing preserves stem cell identity [RNA-Seq] 347 samples
- GSE317764 High-Fat Diet Causes Rapid Loss of Intestinal Group 3 Innate Lymphoid Cells Through Microbiota-Driven Inflammation 270 samples
- GSE281472 Targeting CD206+ macrophages disrupts the establishment of a key anti-tumor immune axis 245 samples
- GSE285826 SenCat: Redefining human cell senescence 232 samples
- GSE342640 Insulin resistance is associated with mammary mitochondrial dysfunction at the onset of human lactation 159 samples
Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.