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Transcriptional control of Sulf1 by WT1 primes epicardial signalling and fate by modifying extracellular heparan sulfate status [ATAC-Seq]

GSE299995 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2026/08/06 Platform GPL19057
Summary
Considering the transcriptional correlations we observed between WT1 and 6-O-endosulfatases in the epicardium, we sought to elucidate whether WT1 regulates 6-O-endosulfatase expression directly and how its binding may differ between Sulf1 and Sulf2 in the epicardium. We first assessed the landscape of chromatin accessibility in FACS sorted epicardial cells at E13.5 (CD31-tdTomato+PDPN+), and employed TOBIAS footprinting to identify candidate WT1 binding sites in the Sulf1 and Sulf2 loci. This analysis predicted WT1 binding to the highly accessible Sulf1 promoter but no binding was predicted for Sulf2. As expected, there was lower accessibility in this locus which reflected reduced Sulf2 gene activity in the epicardial layer.
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Direct links to NCBI, no account and no request form: the whole study as GSE299995_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1278672 and SRA study SRP592603. Searching any of these in the dataset finder brings you back here.

Study design
4 conditions, each sampled once — no replicated groups

Read from 4 sample titles: 4 distinct titles with little repetition. Check it against the sample list below before relying on it.

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