← BioTransfer GEO Dataset Finder
GEO series

RNA-seq analysis of wild-type HEK293, HEK293 treated with DMSO, ML216 or NSC617145, wild-type mESCs and dhx9KO mESCs

GSE254763 Mus musculus; Homo sapiens Expression profiling by high throughput sequencing 15 samples Submitted 2024/07/25 Platform GPL24247Platform GPL24676
Summary
G4s are built by stacked guanine tetrads connected via Hoogsteen hydrogen bonds and can be formed by intra- or inter-molecular folding of the tetramers. R-loops are three-stranded structures which contain a DNA-RNA hybrid and a displaced single-stranded DNA. Both G4s and R-loops are involved in key biological processes, including transcriptional regulation, replication, genomic instability, class switch recombination in B cells, DNA damage and repair, and telomere maintenance. To understand the transcriptional regulation of G4s and R-loops, we performed the RNA-seq analysis on HEK293 cells treated with the inhibitors of G4 resolving helicase ML216 or NSC617145 and mESCs in which the resolving helicase DHX9 of G4 and R-loop was knockout.
Published in
Genome-wide mapping of native co-localized G4s and R-loops in living cells
Liu T, Shen X, Ren Y et al. · eLife 2024 · PMID 39392462 · doi:10.7554/eLife.99026
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE254763_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 15 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1071643 and SRA study SRP487224. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 15 more — browse all 15 samples with per-sample file links →

Similar datasets

Search all RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.