GEO series
Human and mouse adrenal glands are characterized by species-specific steroidogenic states and tissue turnover [scRNA-seq]
GSE253849
Homo sapiens; Mus musculus
Expression profiling by high throughput sequencing
22 samples
Submitted 2026/05/11
Platform GPL24247Platform GPL24676
Summary
The adrenal gland is a fundamental endocrine organ exhibiting constant renewal. Despite extensive work in rodents, human-specific aspects of hormone-producing cell populations, stem cells, and self-renewal mechanisms are not fully understood. To fill this knowledge gap and address species-specific features, we generated high density single-cell and spatial transcriptomics atlases of adult human and mouse adrenal glands using Visium, VisiumHD and Stereo-seq, covering a spectrum of sexes and ages. Our comparisons between human and murine adrenal gland revealed significant evolutionary divergence despite homology in function of specific regions, which questiones the applicability of mouse models in studies of human adrenal disorders. Self-renewal mechanisms in adrenocortical populations that were previously discovered in mice also revealed human-specific features or were confronted by human-specific transitions not evident in rodents. The latter included specific perivascular mesenchymal progenitors, and dispersed SF1+/EZH2+ progenitor cells, which implies that human adrenocortical cells may be renewed in a localized manner in addition to the centripetal model. We also engaged in cross-comparison of our adult human adrenals with fetal stages to reveal developmental strategies still operating as a part of self-renewal in adults. Unexpectedly, human SF1+/EZH2+ progenitors turned out to be operating during adrenocortical development and later during adult stages, albeit in different degrees. At last, we revealed sex-specific differences in human cholesterol metabolism and other features reflecting human and mouse sexual dimorphism in adrenal glands. Overall, our new comparative mouse-human atlases shall provide an additional resource for further molecular interrogations of adrenal gland biology and pathology.
This dataset
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Direct links to NCBI, no account and no request form: the whole study as GSE253849_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 22 samples. Raw sequencing reads are also available from ENA.
Also filed as BioProject PRJNA1067569 and SRA study SRP485201. Searching any of these in the dataset finder brings you back here.
Study design
22 conditions, each sampled once — no replicated groups
Read from 22 sample titles: 22 distinct titles with little repetition. Check it against the sample list below before relying on it.
Samples in this study
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- GSM8028731 Human_adult_adrenal_Female_18_years, healthy adult, scRNAseq
- GSM8028732 Human_adult_adrenal_Female_53_years_1, healthy adult, scRNAseq
- GSM8028733 Human_adult_adrenal_Female_53_years_2, healthy adult, scRNAseq
- GSM8028734 Human_adult_adrenal_Female_59_years, healthy adult, scRNAseq
- GSM8028735 Human_adult_adrenal_Female_64_years, healthy adult, scRNAseq
- GSM8028736 Human_adult_adrenal_Male_39_years, healthy adult, scRNAseq
- GSM8028737 Human_adult_adrenal_Male_48_years, healthy adult, scRNAseq
- GSM8028738 Human_adult_adrenal_Male_52_years, healthy adult, scRNAseq
- GSM8028739 Human_adult_adrenal_Male_54_years, healthy adult, scRNAseq
- GSM8028740 Human_adult_adrenal_Male_57_years, healthy adult, scRNAseq
- GSM8028741 Human_adult_adrenal_Male_73_years, healthy adult, scRNAseq
- GSM8028742 Mouse_adult_adrenal_Female_1, healthy adult, scRNAseq
- GSM8028743 Mouse_adult_adrenal_Female_2, healthy adult, scRNAseq
- GSM8028744 Mouse_adult_adrenal_Female_3, healthy adult, scRNAseq
- GSM8028745 Mouse_adult_adrenal_Female_4, healthy adult, scRNAseq
- GSM8028746 Mouse_adult_adrenal_Female_5, healthy adult, scRNAseq
- GSM8028747 Mouse_adult_adrenal_Mixed_1, healthy adult, scRNAseq
- GSM8028748 Mouse_adult_adrenal_Male_1, healthy adult, scRNAseq
- GSM8028749 Mouse_adult_adrenal_Male_2, healthy adult, scRNAseq
- GSM8028750 Mouse_adult_adrenal_Male_3, healthy adult, scRNAseq
- GSM8028751 Mouse_adult_adrenal_Male_4, healthy adult, scRNAseq
- GSM8028752 Mouse_adult_adrenal_Male_5, healthy adult, scRNAseq
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